attune flow cytometer - An Overview
attune flow cytometer - An Overview
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At the moment, the early indications have been dismissed as particles, nevertheless the retrospective evaluation shown shared properties While using the problematic cells in culture. Tracing the an infection served the lab build added laboratory treatments for screening and defense of assay-important mobile lines.
Whether or not you’re a newbie or an expert, investigate our resources to progress your study and experience in flow cytometry.
Incorporate acoustic concentrating technological innovation with higher-parameter Assessment for flexible spectral unmixing and standard workflows.
Uncover new insights into your experimental facts by visualizing populations employing mixed fluorescence and morphology information. Our software package interface wizards permit downsampling, concatenation (merged data files) and dimensionality applications which can be designed into your software package for an intuitive, streamlined info analysis working experience.
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Also, it can be illuminating to discover the number of doublets slip via conventional singlet gates, and great to verify the position of sudden double-optimistic activities in my Assessment."
Pair your Attune flow cytometer by having an autosampler for greater workflow performance, and include robotic integration for maximum throughput.
Reliable graphic quality even at large throughput Acoustic concentrating and also a significant-velocity camera Blend to picture these T cells consistently at reduced or large flow prices. Effortlessly change emphasis and digicam configurations to satisfy experimental needs.
Early indications of this contamination experienced originally been dismissed as debris. Backgating to find out new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also enables you to use morphological capabilities to find attention-grabbing subpopulations that will not be evident from flow cytometry knowledge on your own.
Users can check out celebration pictures in actual-time to confirm sample high-quality, optimize protocols, and document the morphology of every mobile inhabitants discovered for analysis. Imaging capture also Rewards from acoustic concentrating, which helps to posture the cells in order that a pointy, centered graphic is obtained.
AI-driven automated graphic Evaluation While using the Attune Cytometric Software program translates party characteristics into distinctive morphology parameters which might be coupled with regular fluorescence and scatter parameters to reveal really reproducible inhabitants modifications and special subsets.
We Formerly shown the strength of imaging Motor vehicle-T/Ramos cell interactions. Let’s take a look at just the inhabitants of best curiosity, the double good events, to learn more. We can easily now use extended morphological parameters (below, circularity and skewness of intensity) click here to even more study the attributes of these populations and refine gating on these occasions, growing knowledge robustness.
Designed-in dimensionality reduction tools improved resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to confirm predicted populace resolution in lysed total human blood. Consumer can look at situations from Every cluster for visual confirmation of mobile phenotypes.
Adaptable payment strategy from Agilent Monetary Answers help you obtain the important instrumentation you will need for quick challenge, or to increase your lab.
Gating on these extended parameters means that you can quickly and precisely establish populations of fascination and make sure gating system with little or no guide critique. The Attune program delivers use of condition-of-the-art equipment Discovering Examination instruments in an expertly made user interface, which makes it extremely convenient to use.
The Attune Xenith cytometer is actually a superior-parameter spectral analyzer, furnishing the two spectral unmixing and conventional flow capabilities for thorough and multipurpose cell Evaluation.
The Attune CytPix Flow Cytometer permits end users to visualize samples during acquisition, confirm the precision of gating techniques, and help benefits with reliable documentation for publication applications.
What appeared to be a homogeneous population is definitely more assorted—an insight that needs to be thought of when interpreting outcomes.